26.13 Red cell folate (categories 2)

Red cell folate data available for STOP cohort only.

Over detection limit (360 to 1789 vs >=1790).

26.13.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	180.256937	1	<0.0001	***
rcfolate_gp2	4.092289	1	0.04	*
GA_samp	71.304099	1	<0.0001	***

hPL (μg/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	42.3880663	1	<0.0001	***
rcfolate_gp2	0.2369317	1	0.6	ns
GA_samp	1115.0372969	1	<0.0001	***

GH2 (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	424.0485398	1	<0.0001	***
rcfolate_gp2	0.0362665	1	0.8	ns
GA_samp	553.8496342	1	<0.0001	***

Insulin (uU/ml)

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate_gp2	1	Inf	5.039	5.039	0.02	*
GA_samp	1	Inf	0.370	0.370	0.5	ns

HOMA-IR

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate_gp2	1	Inf	5.570	5.570	0.02	*
GA_samp	1	Inf	0.271	0.271	0.6	ns

Glucose (mmol/L)

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate_gp2	1	Inf	4.972	4.972	0.03	*
GA_samp	1	Inf	1.108	1.108	0.3	ns

26.13.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)

Var	Effect	P
(Intercept)	15.04 (10.13, 22.34)	<0.0001
rcfolate_gp2≥1790	1.14 (1.00, 1.29)	0.04
GA_samp	1.15 (1.11, 1.18)	<0.0001

hPL (μg/ml)

Var	Effect	P
(Intercept)	2.00 (1.62, 2.46)	<0.0001
rcfolate_gp2≥1790	1.02 (0.95, 1.09)	0.63
GA_samp	1.35 (1.33, 1.37)	<0.0001

GH2 (ng/ml)

Var	Effect	P
(Intercept)	0.05 (0.03, 0.06)	<0.0001
rcfolate_gp2≥1790	0.99 (0.90, 1.09)	0.85
GA_samp	1.35 (1.32, 1.38)	<0.0001

Insulin (uU/ml)

Var	Effect	P
(Intercept)	8.46 (5.22, 13.72)	<0.0001
rcfolate_gp2≥1790	1.23 (1.03, 1.47)	0.02
GA_samp	1.01 (0.97, 1.06)	0.54

HOMA-IR

Var	Effect	P
(Intercept)	1.80 (1.09, 2.96)	0.02
rcfolate_gp2≥1790	1.25 (1.04, 1.51)	0.02
GA_samp	1.01 (0.97, 1.06)	0.60

Glucose (mmol/L)

Var	Effect	P
(Intercept)	4.89 (4.46, 5.36)	<0.0001
rcfolate_gp2≥1790	1.06 (1.01, 1.12)	0.03
GA_samp	1.00 (0.99, 1.00)	0.29

26.13.3 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

26.13.4 Estimated marginal means

Outcome	rcfolate_gp2	response	SE	df	lower.CL	upper.CL
PRL (ng/ml)	360 to 1789	72.674	5.958	63.383	61.693	85.609
PRL (ng/ml)	≥1790	82.714	7.863	110.212	68.511	99.861
hPL (μg/ml)	360 to 1789	62.143	1.865	51.975	58.510	66.001
hPL (μg/ml)	≥1790	63.218	2.526	144.272	58.417	68.414
GH2 (ng/ml)	360 to 1789	1.449	0.052	61.530	1.348	1.558
GH2 (ng/ml)	≥1790	1.435	0.075	206.783	1.295	1.590
Insulin (uU/ml)	360 to 1789	9.833	0.351	Inf	9.168	10.545
Insulin (uU/ml)	≥1790	12.070	1.012	Inf	10.241	14.226
HOMA-IR	360 to 1789	2.052	0.077	Inf	1.906	2.210
HOMA-IR	≥1790	2.570	0.224	Inf	2.166	3.050
Glucose (mmol/L)	360 to 1789	4.669	0.039	Inf	4.593	4.747
Glucose (mmol/L)	≥1790	4.954	0.125	Inf	4.715	5.204

26.13.5 Plots

26.13.6 Post-hoc comparisons

Outcome	Contrast	Ratio of Geometric means (95% CI)	P-value
PRL (ng/ml)	≥1790 / 360 to 1789	1.14 (1.00, 1.29)	0.04
hPL (μg/ml)	≥1790 / 360 to 1789	1.02 (0.95, 1.09)	0.6
GH2 (ng/ml)	≥1790 / 360 to 1789	0.99 (0.90, 1.09)	0.8
Insulin (uU/ml)	≥1790 / 360 to 1789	1.23 (1.03, 1.47)	0.02
HOMA-IR	≥1790 / 360 to 1789	1.25 (1.04, 1.51)	0.02
Glucose (mmol/L)	≥1790 / 360 to 1789	1.06 (1.01, 1.12)	0.03