26.13 Red cell folate (categories 2)

Red cell folate data available for STOP cohort only.


Over detection limit (360 to 1789 vs >=1790).

26.13.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 180.256937 1 <0.0001 ***
rcfolate_gp2 4.092289 1 0.04 *
GA_samp 71.304099 1 <0.0001 ***
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 42.3880663 1 <0.0001 ***
rcfolate_gp2 0.2369317 1 0.6 ns
GA_samp 1115.0372969 1 <0.0001 ***
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 424.0485398 1 <0.0001 ***
rcfolate_gp2 0.0362665 1 0.8 ns
GA_samp 553.8496342 1 <0.0001 ***
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
rcfolate_gp2 1 Inf 5.039 5.039 0.02 *
GA_samp 1 Inf 0.370 0.370 0.5 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
rcfolate_gp2 1 Inf 5.570 5.570 0.02 *
GA_samp 1 Inf 0.271 0.271 0.6 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
rcfolate_gp2 1 Inf 4.972 4.972 0.03 *
GA_samp 1 Inf 1.108 1.108 0.3 ns

26.13.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 15.04 (10.13, 22.34) <0.0001
rcfolate_gp2≥1790 1.14 (1.00, 1.29) 0.04
GA_samp 1.15 (1.11, 1.18) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 2.00 (1.62, 2.46) <0.0001
rcfolate_gp2≥1790 1.02 (0.95, 1.09) 0.63
GA_samp 1.35 (1.33, 1.37) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.05 (0.03, 0.06) <0.0001
rcfolate_gp2≥1790 0.99 (0.90, 1.09) 0.85
GA_samp 1.35 (1.32, 1.38) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 8.46 (5.22, 13.72) <0.0001
rcfolate_gp2≥1790 1.23 (1.03, 1.47) 0.02
GA_samp 1.01 (0.97, 1.06) 0.54
HOMA-IR
Var Effect P
(Intercept) 1.80 (1.09, 2.96) 0.02
rcfolate_gp2≥1790 1.25 (1.04, 1.51) 0.02
GA_samp 1.01 (0.97, 1.06) 0.60
Glucose (mmol/L)
Var Effect P
(Intercept) 4.89 (4.46, 5.36) <0.0001
rcfolate_gp2≥1790 1.06 (1.01, 1.12) 0.03
GA_samp 1.00 (0.99, 1.00) 0.29

26.13.3 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

26.13.4 Estimated marginal means

Outcome rcfolate_gp2 response SE df lower.CL upper.CL
PRL (ng/ml) 360 to 1789 72.674 5.958 63.383 61.693 85.609
PRL (ng/ml) ≥1790 82.714 7.863 110.212 68.511 99.861
hPL (μg/ml) 360 to 1789 62.143 1.865 51.975 58.510 66.001
hPL (μg/ml) ≥1790 63.218 2.526 144.272 58.417 68.414
GH2 (ng/ml) 360 to 1789 1.449 0.052 61.530 1.348 1.558
GH2 (ng/ml) ≥1790 1.435 0.075 206.783 1.295 1.590
Insulin (uU/ml) 360 to 1789 9.833 0.351 Inf 9.168 10.545
Insulin (uU/ml) ≥1790 12.070 1.012 Inf 10.241 14.226
HOMA-IR 360 to 1789 2.052 0.077 Inf 1.906 2.210
HOMA-IR ≥1790 2.570 0.224 Inf 2.166 3.050
Glucose (mmol/L) 360 to 1789 4.669 0.039 Inf 4.593 4.747
Glucose (mmol/L) ≥1790 4.954 0.125 Inf 4.715 5.204

26.13.5 Plots

26.13.6 Post-hoc comparisons

Outcome Contrast Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) ≥1790 / 360 to 1789 1.14 (1.00, 1.29) 0.04
hPL (μg/ml) ≥1790 / 360 to 1789 1.02 (0.95, 1.09) 0.6
GH2 (ng/ml) ≥1790 / 360 to 1789 0.99 (0.90, 1.09) 0.8
Insulin (uU/ml) ≥1790 / 360 to 1789 1.23 (1.03, 1.47) 0.02
HOMA-IR ≥1790 / 360 to 1789 1.25 (1.04, 1.51) 0.02
Glucose (mmol/L) ≥1790 / 360 to 1789 1.06 (1.01, 1.12) 0.03